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GAZZETTA MEDICA ITALIANA ARCHIVIO PER LE SCIENZE MEDICHE
A Journal on Internal Medicine and Pharmacology
Indexed/Abstracted in: BIOSIS Previews, EMBASE, Scopus, Emerging Sources Citation Index
Gazzetta Medica Italiana Archivio per le Scienze Mediche 2009 August;168(4):241-5
Urine particles examination: comparison of automated flow citometer UF-1000i and manual quantitative microscopy
Manoni F., Ferrian M., Gusella G., Ercolin M., Tinello A., Fornasiero L., Valverde S., Gessoni G.
1 Clinical Pathology Department, Ospedale Civile Monselice, Pordenone, Italy
2 Service of Laboratory Medicine, Ospedale Civile Chioggia, Venezia, Italy
Aim. Some automated instruments examining urine sediment have been introduced. We compared the performance of Sysmex UF-1000i with manual microscopy in urine particles examination.
Methods. 15 urine samples were selected. Urine sediments were examined by manual microscopy by using Fuchs-Rosenthal chamber and UF-1000i analyzer.
Results. The within-run CVs for urine samples ranged from 0.8% to 23.1% for the UF-1000i. The agreement between methods was good for red blood cells and white blood cells counts based on r values of 0.99, for epithelial cell the r value was 0.98 and 0.87 for casts.
Conclusion. This automated urinalysis system demonstrated good concordance with manual quantitative microscopy. Thus, when combined with urine chemistry analysis, this analyzer might provide a rapid and accurate screening tool in routine urine analysis.