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Indexed/Abstracted in: BIOSIS Previews, EMBASE, Scopus, Emerging Sources Citation Index
Online ISSN 1827-1812
Nazem H. 1, Fazilati M. 2
1 Payame Nour University of Iran Esfahan, Iran;
2 Isfahan University of Technology, Isfahan, Iran
In this research project, effect of halothane on activity of glucokinase enzyme in serum and hepatic tissue using two different procedures of assessing glucose-6- phosphate dehydrogease and coenzyme NAD+ Vs NADP+ was compared. Twenty-four hours following inhalation of halothane, activity of glucokinase enzyme vs control group showed a significant difference (p<0.5), while enzyme activity produced by glucose -6- phosphates dehydrogenaze obtained from fungus Leuco-nostoe mesenteries and coenzayme NAD+ shows a greater difference compared with controls. Therefore it can be concluded that methodology of assessing glucokinase enzyme and level of precision and sensitivity of employed procedure can effect determination of halothane effect on hepatic tissue following increased activity of glucokinase enzyme. Therefore conduction of all studies and investigations of human and the on male subjects, and use of precise proposed procedure for assessment of this enzyme show that such studies showed be conducted on other enzyme. On the other hand, comparison of these two methods can provides an appropriate model for minimum drug allergy in particular to halothane in man or animals.