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Togni S. 1, Maramaldi G. 1, Meneghin M. 1, Giacomelli L. 2, Cattaneo R. 3, Eggenhoffner R. 2, Burastero S. 4
1 Indena S.p.A, Milan, Italy;
2 Department of Surgical Sciences and Integrated Diagnostics, School of Medicine, Genova University, Genoa, Italy;
3 Abich, Verbania, Italy;
4 San Raffaele Scientific Institute, Milan, Italy
AIM: Quercetin is a very abundant flavonoid that has many potential benefits for human health, especially for its antioxidant, anti-inflammatory and antiallergic properties. In this study we present two in vitro laboratory experiments performed to assess the antioxidative and antiallergic properties of quercetin.
METHODS: In the first experiment the effect of quercetin on UV-induced oxidative damage was tested on a stabilized human keratinocytes cell line, using a fluorogenic probe (dichloroflurescein diacetate) to measure the concentration of reactive oxygen species (ROS) inside the cells. Of note, we used a particular formulation of quercetin, characterized by a phospholipids based delivery system that increases the ability of quercetin to permeate the skin. In the second experiment we evaluated the ability of quercetin phospholipids to inhibit basophils degranulation on rat basophil cells expressing the human receptor FcεRI. The positive control was obtained by cross-linking the receptor FcεRI with a specific policlonal antibody; in addition the effect of quercetin was compared to that of hyaluronic acid (HA) which is capable to inhibit the effect of the polyclonal antibody.
RESULTS: The first experiment showed that quercetin phospholipids 1% was able to reduce ROS formation caused by UVA exposure in a dose-dependent manner and in response to irradiation levels ranging from 1 to 5 J. The second experiment revealed that quercetin was able to remarkably reduce basophils degranulation in a dose-dependent manner and at similar levels if compared to the positive control (HA).
CONCLUSION: The results of our in vitro experiments provide further evidence on the ability of quercetin to protect the cells against various cellular insults, thanks to its antioxidant and antiallergic properties.