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Official Journal of the Italian Society of Dermatology and Sexually Transmitted Diseases
Indexed/Abstracted in: EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,014
Giacomo CALDAROLA 1, Angelo CARBONE 1, Vincenzo ARENA 2, Ilaria PENNACCHIA 2, Chiara DE WAURE 3, Giovina VIANALE 4, Franco SCALDAFERRI 5, Magda D’AGOSTINO 1, Francesco VALENZANO 1, Erica COSTANTINI 4, Matteo AURIEMMA 4, Paolo AMERIO 6, Clara DE SIMONE 1
1 Institute of Dermatology, “Sacro Cuore” Catholic University, Rome, Italy; 2 Institute of Pathology, “Sacro Cuore” Catholic University, Rome, Italy; 3 Institute of Hygiene, “Sacro Cuore” Catholic University, Rome, Italy; 4 Department of Experimental and Clinical Sciences, G. d’Annunzio University, Chieti, Italy; 5 Division of Gastroenterology, Department of Internal Medicine, “Sacro Cuore” Catholic University, Rome, Italy; 6 Dermatology Clinic, Department of Medicine and Aging Science (DMSI), G. d’Annunzio University, Chieti, Italy
BACKGROUND: Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) is a cytokine member of the tumour necrosis factor (TNF) family. Its role has been investigated in skin cancers and some inflammatory and/or immune-mediated skin diseases. An involvement of TRAIL in psoriasis pathogenesis has recently been hypothesized. We investigated the expression and localization of TRAIL and its receptors in psoriatic skin and measured serum TRAIL. The intracellular pathways activated by TRAIL were assessed to investigate its potential role in the pathogenesis of psoriasis.
METHODS: Twenty-four consecutive patients with plaque psoriasis and age- and sex-matched healthy subjects were recruited. Serum TRAIL was measured by means of an enzyme-linked immunosorbent assay (ELISA). TRAIL and TRAIL receptors were evaluated by reverse transcription – polymerase chain reaction (RT-PCR) (RNA of lesional and non-lesional psoriatic skin) and by immunohistochemistry (lesional skin). Caspase 8 and NF-kB immunoexpression were also evaluated by immunohistochemistry.
RESULTS: RT-PCR demonstrated increased synthesis of TRAIL and its receptors in lesional vs. non-lesional skin. Immunohistochemistry showed a strong staining of TRAIL and TRAIL receptors both in the epidermis and in the dermal infiltrate. Finally, a correlation emerged between caspase 8 and TRAIL immunoexpression in the dermis.
CONCLUSIONS: Our findings suggest an involvement of TRAIL in psoriasis pathogenesis, probably through an action at the site of the inflammatory infiltrate, likely via caspase 8.