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GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA
A Journal on Dermatology and Sexually Transmitted Diseases
Official Journal of the Italian Society of Dermatology and Sexually Transmitted Diseases
Indexed/Abstracted in: EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,014
Giornale Italiano di Dermatologia e Venereologia 2014 August;149(4):427-33
Effect of intradermal injection of heparinase III on skin wound healing in diabetic rats
Yousefimanesh H. 1, Abdolahi F. 1, Jahromi B. M. 1, Bagheri M. 1, Noorafshan A. 2, Zarifkar A. 3, 4, Mirkhani H. 5, 6 ✉
1 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran;
2 Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;
3 Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;
4 Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;
5 Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;
6 Medicinal and Natural Products Chemistry, Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
AIM: neuropathy and vascular damage in this disease. Heparanase is an endoglycosidase that degrades heparan sulfate in the extracellular matrix and is believed to promote angiogenesis. The present study has been performed to investigate the effect of heparinase III (an enzyme which exclusively cleaves heparan sulfate) on wound healing in diabetic rats.
METHODS: The rats became diabetic by a single streptozotocin injection. Two weeks later, a wound was created by excision of the skin in the left paravertebral area. Heparinase III (0.2 unit) was injected intradermally around the wound every 5 days, starting on day one, for a total of three doses. The wound area was measured every 3 days. After completion of wound healing, full thickness skin samples were taken from the wound sites and evaluated for volume density of the collagen bundles, numerical density of the fibroblasts, and length density of the vessels.
RESULTS: Heparinase III accelerated wound closure compared to control diabetic animals. Microscopical examination revealed that it increased angiogenesis with no significant effect on collagen density and the number of fibroblasts.
CONCLUSION: Heparinase III induces angiogenesis and improves wound healing in diabetic animal model.