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GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA
A Journal on Dermatology and Sexually Transmitted Diseases
Official Journal of the Italian Society of Dermatology and Sexually Transmitted Diseases
Indexed/Abstracted in: EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,014
Giornale Italiano di Dermatologia e Venereologia 1998 October;133(5):329-32
Evaluation of the antifungal effect of naftifine by neutral red staining
Campanile G., Del Bianco E., Difonzo E. M.
Università degli Studi - Firenze, Istituto di Clinica Dermosifilopatica
Background. Naftifine, a new antifungal agent belonging to the allylamine group, possesses a high degree of in vitro antifungal activity, with Minimal Inhibitory Concentration (MIC) values for dermatophytes of 0.05 to 0.2 mg/l. Its antifungal effect has been evaluated in vitro using neutral red staining, a newly described method for evaluating the viability of fungal cells.
Methods. A conidial suspension of Trichophyton mentagrophytes was treated with various concentrations of naftifine. After 24, 48, 72, 96 and 120 hrs of incubation, ten microlitres of each suspension were used for neutral red staining, calculating the percentage of positive fungal elements, and 50 microlitres were subcultured.
Results. Suspensions treated with naftifine showed a rapid decrease in the percentage of neutral red positive fungal elements. This decrease was already evident at the first observations for the suspensions treated with the highest drug concentrations, which at 96 and 120 hrs did not present any viable elements. Strong positive correlations were found between the number of positive fungal elements and the number of subsequent subcultures. After 72 hrs the suspension treated with 5
µg/ml did not show any culture.
Conclusions. Our results confirm that naftifine exercises an antifungal effect also at concentrations of drug lower than those indicated by MIC values and that neutral red staining is a useful method for evaluating the antifungal effect of a drug.