Total amount: € 0,00
Official Journal of the Italian Society of Dermatology and Sexually Transmitted Diseases
Indexed/Abstracted in: EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,014
Online ISSN 1827-1820
Campanile G. 1, Ludovici M. 2, Bianchi B. 1, Difonzo E. M. 1
1 Università degli Studi - Firenze, Istituto di Clinica Dermosifilopatica;
2 Dipartimento di Farmacologia Preclinica e Clinica
Background. The accumulation of neutrophils in the infected skin is an early event during dermatomycosis. For this reason, the potential antiphlogistic activity of an antifungal drug, especially topical, supports its therapeutical effect very much, in particular in case of very phlogistic dermatophytoses. The antinflammatory activity of naftifine is studied, investigating its influence on the leukotriene B4 metabolism of human neutrophil granulocytes (PMN).
Methods. The cells were stimulated with the Ca-ionophore A23187 over 15 minutes in the presence of the drug. Subsequently the supernatants were analyzed for their leukotriene B4 content by reversed-phase HPLC.
Results. The results showed that naftifine inhibited the release of leukotriene B4 in PMN already at concentrations of 6.4 µ/ml. At concentrations of 640 µ/ml naftifine totally blocked the release of leukotrien B4 in PMN.
Conclusions. Further investigations should clarify what enzymes are important for the observed antinflammatory activity. The interaction with cytochrome-P450 by azoles cannot be accepted for naftifine which does not act at this level.