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Indexed/Abstracted in: EMBASE, Scopus, Emerging Sources Citation Index
Online ISSN 1827-1782
Wu H., Iwata H., Kimura M., Sekino T., Yamada T., Takemura H.
Department of General and Cardiothoracic Surgery, Graduate School of Medicine, Gifu University, Gifu, Japan
AIM: Shigyaku-san is known to alleviate the effects of experimental liver damage. The aim of this study was to clarify the effects of Shigyaku-san on liver function and regeneration after hepatectomy in rats.
METHODS: Rats were divided into three groups (control, Shigyaku-san, no-hepatectomy). Blood biochemistry was analyzed at 2, 6, 24, and 72 h postoperatively. The remnant liver, liver-body weight ratio and immunohistochemistry stains were evaluated before hepatectomy and at 24 and 72 h postoperatively. Serum IL-6 and TNF-α were measured before hepatectomy and 2 and 6 h postoperatively.
RESULTS: Serum ALT and T-Chol were significantly lower in the Shigyaku-san group than in the control group at 24 and 72 h postoperatively, and serum LDH was significantly lower 6 h postoperatively. Regenerated liver weight was significantly increased in the Shigyaku-san group compared with the control group at 72 h postoperatively. The proliferating cell nuclear antigen (PCNA) labeling index (LI) and liver-body weight ratio were significantly higher in the Shigyaku-san group than in the control group 24 and 72 h postoperatively. Ki67 was significantly higher in the Shigyaku-san group than in the control group 72 h postoperatively. Compared with the control group, serum IL-6 was significantly increased 2 h postoperatively, but significantly decreased 6 h postoperatively in the Shigyaku-san group.
CONCLUSION: These data suggest that Shigyaku-san protects against liver damage and promotes liver regeneration after partial hepatectomy in rats.