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CURRENT ISSUETHE JOURNAL OF CARDIOVASCULAR SURGERY

A Journal on Cardiac, Vascular and Thoracic Surgery

Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,632

Frequency: Bi-Monthly

ISSN 0021-9509

Online ISSN 1827-191X

 

The Journal of Cardiovascular Surgery 2003 February;44(1):1-7

CARDIAC SECTION 

    ORIGINAL ARTICLES

Myocardial extracellular signal regulatory kinases are activated by laser treatment

Liu X. H. 1,2, Grund F. 1, Kanellopoulos G. K. 1,3, Kvernebo K. 1,4, Ilebekk A. 1

1 Institute ­for Experimental Medical Research Ullevål University Hospital, Oslo, Norway
2 Institute of Geriatric Cardiology Chinese ­PLA General Hospital, Beijing, China
3 Department of Cardiothoracic Surgery, Glenfield Hospital, Leicester, UK
4 Department of Cardio-tho­rac­ic Surgery Ullevål University Hospital, Oslo, Norway

Aim. Transmyocardial ­laser revas­cu­lar­iza­tion (­TMR) ­and per­cut­ane­ous trans­lu­mi­nal myo­car­dial ­laser revas­cu­lar­iza­tion (­PTMR) ­have ­anti-angi­nal ­effects on cer­tain ­groups of ­patients ­with ischem­ic ­heart dis­ease, pos­sibly by induc­ing myo­car­dial neo­an­gio­gen­e­sis ­through ­the mechan­i­cal inju­ry. Here we exam­ine ­the ­effects of ­TMR ­and ­PTMR on extra­cel­lu­lar sig­nal reg­u­la­to­ry kinas­es (ERKs), ­which ­have ­been impli­cat­ed in ­the con­trol of neo­an­gio­gen­e­sis in ­vitro.
Methods. Eight ­pigs ­were anes­the­tized ­with ket­a­mine ­and fen­ta­nyl. In ­five ­pigs ­TMR ­was per­formed in ­the ­left ven­tric­u­lar ante­ri­or ­wall ­and ­PTMR in ­the pos­te­ri­or ­wall. Biopsies ­were tak­en ­either ­after 2 ­hours, 3, 7, 14, ­and 30 ­days. Three ­pigs ­served as con­trols ­and pro­vid­ed sam­ples ­for base­line val­ues ­and ­time-­matched con­trols at 2 ­hours ­and 3 ­days. ERKs activ­ity ­was deter­mined by ­increased phos­phor­y­la­tion of mye­lin ­basic pro­tein. Total ERKs pro­tein abun­dance ­was deter­mined by Western ­blot ­with an anti­body ­against ­ERK1 ­and ­ERK2.
Results. It ­was ­found ­that ERKs activ­ity ­was high­er in ­all sam­ples ­from ­the ­laser treat­ed myo­car­di­um ­than in ­the con­trol sam­ple at base­line (­TMR: ≥1878 ­pmol Pi. ­min-1 mg pr-1 vs 104 ­pmol Pi. ­min-1 mg pr-1, respec­tive­ly, p<0.05. ­PTMR: ≥346 ­pmol Pi. ­min-1 mg pr-1 vs 136 ­pmol Pi ­min-1 mg pr-1, respec­tive­ly, p<0.05). The ­time-­matched sam­ples ­showed ­increased activ­ities of ERKs ­after ­laser treat­ment. The pro­tein lev­el of ERKs in myo­car­di­um treat­ed ­with ­TMR ­and ­PTMR par­allelled ­the ­data on ERKs activ­ity.
Conclusion. Our ­data sug­gest ­that ­the ERKs ­system is acti­vat­ed in ­the por­cine ­heart by ­the mechan­i­cal inju­ry of ­TMR ­and ­PTMR.

language: English


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