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The Journal of Cardiovascular Surgery 2000 April;41(2):171-4

Copyright © 2009 EDIZIONI MINERVA MEDICA

language: English

Association of osteopontin with calcification in human mitral valves

Canver C. C., Gregory R. D., Cooler S. D., Voytovich M. C.

From the Division of Cardiothoracic Surgery Albany Medical College, Albany, New York *Department of Pathology University of Wisconsin School of Medicine Madison, Wisconsin, USA


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Back­ground. ­Little is ­known ­about the ­long-­standing inflam­ma­tion ­leading to cal­cifi­ca­tion ­within ­heart ­valves. Oste­o­pontin, a phos­phor­y­lated gly­co­pro­tein, is ­present ­within ath­e­rom­a­tous cal­cific ­plagues in ­response to vas­cular endo­the­lial ­injury. The pur­pose of the ­study was to inves­ti­gate ­whether oste­o­pontin ­exists in cal­cific ­mitral ­valve leaf­lets in ­human ­beings, and to deter­mine a ­link ­between ­chronic inflam­ma­tion ­leading to ­mitral sten­osis and the oste­o­pontin ­status of ­mitral val­vular ­tissue.
Mate­rials and ­methods. We ­reviewed the ­case his­to­ries of 17 ­patients who under­went ­mitral ­valve replace­ment ­therapy for ­mitral sten­osis ­between 1995 and 1997 (8 men and 9 ­women, ­mean age 61 ­years). ­Hybrid ­mouse mono­clonal immu­no­glob­ulin G1 anti­bodies ­were ­used for immu­no­his­to­chem­ical detec­tion of oste­o­pontin in the ace­tone-­fixed spec­imen. The con­trol ­group con­sisted of ­normal ­mitral ­valve ­tissue ­from car­di­om­yo­pathy ­patients who under­went car­diac trans­plan­ta­tion.
­Results. A ­weak oste­o­pontin immu­no­reac­tivity was ­present in appar­ently ­normal ­mitral ­valve ­tissue ­obtained ­from car­di­om­yo­pathy ­patients. All ­mitral sten­osis ­patients had immu­no­reac­tivity (17/17) for oste­o­pontin ­within cal­cific ­deposits of ­mitral ­valve ­tissue. The inten­sity of oste­o­pontin ­activity had a ­strong asso­ci­a­tion ­with ­increasing mac­ro­phage and cal­cium aggre­ga­tions in the ­mitral val­vular ­tissue. We ­found no cor­re­la­tion ­between oste­o­pontin ­status and clin­ical fea­tures on the prog­nosis of cal­cific ­mitral sten­osis.
Con­clu­sions. We con­clude ­that oste­o­pontin coex­ists ­with ­intimal mac­ro­phages in cal­cific ­human ­mitral ­valve ­tissue. Dem­on­stra­tion of ­such asso­ci­a­tion ­between the pres­ence of oste­o­pontin and cal­cifi­ca­tion in ­human ­mitral ­valves is con­sis­tent ­with the hypoth­esis ­that cal­cifi­ca­tion in ­this ­tissue is, at ­least in ­part, an ­actively medi­ated phe­nom­enon.

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