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A Journal on Cardiac, Vascular and Thoracic Surgery

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The Journal of Cardiovascular Surgery 1999 June;40(3):435-8

language: English

Surviving and pro­life­rat­ing ­faculty of fibro­blasts cul­tured ­under no-ser­um and ­poor nutri­tion­al con­di­tions. The ­basic ­study on hybridation of cul­tured ­cells ­with an arti­fi­cial ­matrix

Inoue H., Osada H.*, Kojima K.*, Tomiola M., Ishida H.

From the Department of Plastic and Reconstructive Surgery * Department of Surgery, Division of Respiratory Surgery St. Marianna University School of Medicine Miyamae, Kawasaki, Japan


Background. In ­this ­study, we meas­ured sur­vi­val peri­ods of fibro­blasts cul­tured ­under con­di­tions pro­vid­ing min­i­mal nutri­tion. This inves­ti­ga­tion ­aims to devel­op the ­high affin­ity ­hybrid arti­fi­cial ­organs on tis­sues or ­organs final­ly.
Methods. Dermal fibro­blasts ­were inoc­u­lat­ed on 96-­well mul­ti­plate accord­ing to rou­tine tech­nique. The medi­um of ­these ­cells main­tained for 24 ­hours ­replaced the new DME medi­um con­tain­ing 10% FBS (­group 1) or with­out FBS (­group 2) and ­then cul­tured for 10 ­days with­out medi­um ­change. The sur­viv­ing ­cell num­bers ­were meas­ured ­using MTS ­assay.
Results. Surviving ­cell num­bers of ­both ­groups ­decreased pro­gres­sive­ly dur­ing the 10-day ­assay peri­od. Overall 25-40% of the fibro­blasts sur­vived for at ­least 10 ­days. It ­decreased sig­nif­i­cant­ly, how­ev­er, in ­group 2 ­from day 2 ­through day 4 as com­pared to ­group 1. In con­trast, the ­cell num­ber ­decreased in ­group 1 to a ­greater ­extent ­than in ­group 2 ­after day 6. When the ­cells ­that sur­vived ­until day 10 ­were ­then cul­tured ­with ­fresh DME medi­um con­tain­ing 10% FBS for 3 ­more ­days, the ­cell num­bers ­returned to 50-100% of the ­initial lev­el.
Conclusions. These ­results sug­gest ­that fibro­blasts ­cell func­tion is main­tained ­under ­poor nutri­tion­al con#­ditions, i.e., with­out ser­um, pos­sibly via an auto­crine or par­a­crine mech­a­nism. These ­data ­could con­trib­ute ­much to the ­research of ­hybrid arti­fi­cial ­organs, includ­ing an arti­fi­cial tra­chea, in ­which hybrid­iza­tion of cul­tured ­cells ­with arti­fi­cial matric­es ­remains to be a ­basic pro­cess, and has to ­serve in blood­less post­im­plan­ta­tion peri­od.

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