Home > Journals > Acta Vulnologica > Past Issues > Acta Vulnologica 2014 June;12(2) > Acta Vulnologica 2014 June;12(2):71-80

CURRENT ISSUE
 

ARTICLE TOOLS

Reprints

ACTA VULNOLOGICA

A Journal on Physiopathology and Therapy of Chronic Cutaneous Ulcers


Official Journal of the Italian Association for Cutaneous Ulcers
Indexed/Abstracted in: EMBASE, Scopus, Emerging Sources Citation Index


eTOC

 

ORIGINAL ARTICLES  


Acta Vulnologica 2014 June;12(2):71-80

Copyright © 2014 EDIZIONI MINERVA MEDICA

language: English, Italian

Comparison between in vitro activity of silver sulfadiazine alone and associated with hyaluronic acid against bacteria and fibroblasts

Figura N. 1, Biagi M. 2, Collodel G. 3, Gonnelli S. 1, Moretti E. 3

1 Department of Medical and Surgical Sciences and Neuroscience, Siena University, Policlinico Santa Maria alle Scotte, Siena, Italy; 2 Department of Physics Earth and Environmental Sciences, Siena University, Policlinico Santa Maria alle Scotte, Siena, Italy; 3 Department of Molecolar Medicine and Development, Siena University, Policlinico Santa Maria alle Scotte, Siena, Italy


PDF  


AIM: Pressure and burn ulcers easily undergo superinfection with bacteria colonizing the epidermis. Silver sulfadiazine (SS) 1% solution and silver sulfadiazine with 0.2% hyaluronic acid (SSHA) are considered the gold standard for topic treatment and prevention of bacterial superinfection.
METHODS: Staphylococcus aureus and Pseudomonas aeruginosa were used to determine the in vitro minimum bactericidal concentrations (MBCs) of these compounds, using broth dilution method and subculture onto agar. The potential effects of SS and SSHA upon cell proliferation (FP) was verified using primary human fibroblasts in vitro. Finally, suspensions of S. aureus and P. aeruginosa were examined by transmission electron microscope (TEM) at SS and SSHA MBCs.
RESULTS: MBCs of SS and SSHA were 1:10 (corresponding to 1 mg/mL of SS) and 1:5, respectively, towards all species and the variant tested. FP was significantly reduced by SS and SSHA, but the difference between the two substances was not significant. Bacteria treated with SS showed, at TEM examination, cell membrane alterations, cytoplasm vacuolization and intracytoplasm silver precipitates. Under the effect of SSHA, the bacterial alterations were similar, but less marked.
CONCLUSION: SS showed an increased antibacterial activity respect to SSHA, as also confirmed by TEM. Fibroblast proliferation was decreased by both the compounds, but the difference between them was not significant.

top of page

Publication History

Cite this article as

Corresponding author e-mail